Quantitative Analysis of Fatty Acids from Safflower by Selective and Sensitive Pre-Column Derivatization Method Using HPLC-FLD and Online APCI/MS
نویسندگان
چکیده
منابع مشابه
Analysis of Plasma Amino Acids Using RP-HPLC and Pre-Column Derivatization with OPA/3-MPA
ABSTRACT Background and Objective: Measurement of amino acids is an important tool for metabolic studies and evaluation of patients’ clinical condition. The aim of this study was to analyze the plasma amino acids using reverse phase high performance liquid chromatography techniques (RP-HPLC) with pre-column derivatization by o-phthalaldehyde (OPA)...
متن کاملHPLC-FLD for the simultaneous determination of primary and secondary amino acids from complex biological sample by pre-column derivatization.
A rapid, sensitive, and reproducible pre-column derivatisation procedure has been established for the simultaneous determination of 20 amino acids by high-performance liquid chromatography using fluorescence detection. The amino acids were derivatized using o-phthalaldehyde and 9-fluorenylmethyl-chloroformate reagents. The optimal conditions for simultaneous separation and detection of both pri...
متن کاملanalysis of plasma amino acids using rp-hplc and pre-column derivatization with opa/3-mpa
abstract background and objective: measurement of amino acids is an important tool for metabolic studies and evaluation of patients’ clinical condition. the aim of this study was to analyze the plasma amino acids using reverse phase high performance liquid chromatography techniques (rp-hplc) with pre-column derivatization by o-phthalaldehyde (opa) in combination with 3- mercaptopropionic...
متن کاملA Sensitive Liquid Chromatographic Method for the Analysis of Clarithromycin with Pre-Column Derivatization: Application to a Bioequivalence Study
Objective(s) A sensitive liquid chromatographic method for the analysis of clarithromycin- a macrolide antibiotic- in human serum, using pre-column derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) is described. Materials and Methods The method involved liquid-liquid extraction of the drug and an internal standard (amantadine) followed by pre-column derivatization of the analytes...
متن کاملQuantitative analysis of sialic acids in Chinese conventional foods by HPLC-FLD
Background: Sialic acids are a family of ninecarbon sugar compounds with carboxylic acyl derivatives. It exists in bacteria, fish, mammals and other living organisms, participates in and regulates many important life events, such as cell recognition, membrane flow, endocytosis and so on. Sialic acid is usually located in the outermost layer of the sugar part of the cell membrane and the key pos...
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ژورنال
عنوان ژورنال: Asian Journal of Chemistry
سال: 2015
ISSN: 0970-7077,0975-427X
DOI: 10.14233/ajchem.2015.17953